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Chris Kurz MD, Majid Moshirfar MD
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Drs. Moshirfar and Kurz share their DMEK technique, including donor preparation, unrolling Descemet's membrane, and positioning the graft in the recipient's eye.
Posted: 12/17/2008
Chris Kurz MD, Majid Moshirfar MD
Drs. Moshirfar and Kurz share their DMEK technique, including donor preparation, unrolling Descemet's membrane, and positioning the graft in the recipient's eye.
Posted: 12/17/2008
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Comments
Just Now
12 years ago
I'm not Dr. Kurz, but I can answer the previous poster's questions. The trypan will diffuse out of the graft if left in BSS or optisol for any significant period of time. It does this during surgery as well. After harvesting the graft, I leave it to soak in trypan blue on the corneoscleral rim until it is time to load it in the injector. No, trypan blue is not toxic to the endothelium, or at most very very weakly toxic. Methylene blue, however is. I stopped injecting an air bubble when using trypan blue to stain anterior capsules in mature cataracts after I realized this. DMEK grafts (all of which were soaked in trypan blue) often can have cell counts in the high 2000s.
Omar Barrada
13 years ago
Why do stain the graft repetitively? Isn't staining one sufficient? And, is trypan blue toxic in any way to the endothelium? Thank you.
M S
14 years ago
Descemet's is very elastic, so the graft always folds Descemet's side
HUMBERTO ESCORCE
16 years ago
How do you know what is the endotelial and descemet side to implant
chris kurz
16 years ago
escorce h-
Mehdi Ghajarnia
16 years ago
Fantastic job Drs. Moshirfar and Kurz!
JANNES TAN
16 years ago
HOW MANY ENDOTHELCELL COUNT POSTOPERATIVE?
David de la Hera Vegas
16 years ago
Fantastic
Jonathan Ellis
16 years ago
Nice to see you making history, Chris! Jon Ellis
THEODOSIOS KONTOS
16 years ago
Very Good
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